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Slide #31 : Simple columnar epithelium (caprine gall bladder)
Observe the section with the naked eye and note a dark purple line on a side of the section: this will resolve into epithelial lining. Now locate at 4x the lining (upper region of the section) of the bladder. At 40x, the columnar calls are extremely tall and slender standing upright like columns. The nuclei are elongated and are located in the basal part of the cell. The nuclei are at approximately the same level. Striated border that is not prominent. |
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A large artery is seen surrounded by other lung structures. Look for the blood vessels to be filled with pink, solid staining material, blood (not shown here). Examine microscopically the lining cells of the vessels. At 10x, the endothelium may be observed as a thin strand (cytoplasm) with thickenings (nuclei) at irregular intervals. At 40x, the cells appear as flat dark nuclei joined by a thin strand of pink cytoplasm.
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Slide #22 : Simple, straight tubular gland (caprine caecum) At 4x, locate the lining of the organ. At 10x search for longitudinally-cut tubules which resemble the fingers of a glove. These are the intestinal glands of Lieberkühn. At 40x, note the epithelium is made up of simple columnar and goblet cells. Columnar cells of the gland are continuous with the surface epithelium. Goblet cells exhibit pale basophilia in the apical ends. The cells produce mucin which, following hydration, becomes mucus. Wandering dark cells (lymphocytes) are plentiful in the epithelium. |
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Slide #92 : Unicellular gland - goblet cell (ovine trachea) At 4x, search the lumen of the organ; then at 40x focus on the lining of the lumen. Observe the ciliated pseudostratified columnar epithelium. The goblet cell, which is found among the epithelium, has an expanded apical or distal end and a slender basal or proximal end. The apical end appears to be filled with vacuoles (40x) due to the dissolution of the mucigen droplets. The proximal end contains a compressed nucleus. The term goblet cell is descriptive of the form of the cell which has a cup-shaped distal part, and a proximal part like the stem of a goblet. |
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| Slide #65 : Simple, coiled tubular gland (ovine skin) At 10x examine from left to right the mid-region of the section. Note the large round or elongated holes, some of which may appear to be placed one upon the other. The holes represent the coiled secretory portion of the gland. At 40x, note the lining secretory cells which are cuboidal to columnar. In a few cross-cut segments, the apices of some lining cells show characteristic blebs. Flat cells represent the post-secretory phase of the cycle. The nuclei are round or ovoid. Myoepithelial cells are represented by elongated, plumpy nuclei lying between secretory cell nuclei and basal lamina with their longitudinal axes parallel to the basal lamina.The duct is lined by two layers of small cuboidal epithelium. The lumen of the duct is narrower than the secretory portion. Theoretically, the secretory portion of the gland is loosely rolled into a ball (coiled) hence discontinuities, seen earlier as holes. |
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Slide #40 : Simple, branched tubular gland (canine stomach)
At 4x, focus on the top surface of the section. This is the inner lining of the organ which contains the cardiac gland. At 10x, examine the cross, oblique and longitudinal sections of the gland tubule segments. The lining cells are columnar with clear cell outlines, faint-blue cytoplasm and concave or round nuclei. Theoretically, the duct is unbranched but the secretory portion is branched.
Slide #8 : Compound, acinar mucous gland (canine trachea)
At 4x, located the pale-blue areas, below the ciliated pseudostratified epithelium, which are the mucous glands. We use this slide to study the esophagus. Notice that the cell outlines are distinct. The cytoplasm in foamy or granular and basophilic. Nuclei are condensed and located at the base of the cell.
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Review epithelium |