Nerve trunk (equine).
Note that the osmium stain has only partially penetrated.
The intersection of osmium stained and non-stained nerve axons. This is an excellent place to look for myelinated nerves.
The osmium has stained the myelin, giving it a dark brown appearance. Note the large and small myelinated nerve axons.
Although the myelin cannot be seen, there is an empty space or cart-wheal appearance around the axons of myelinated nerves.